Transgenic Model Design and Generation
Support for Transgenic Model DESIGN and Strategy
Recent developments in the field or transgenic techniques means that sophisticated mouse models can be now generated rapidly and with relative ease. The CTM’s mission is to assist researchers by not only providing cutting-edge transgenic services but to also advise them and if desired carry out all steps of transgenic model design and generation on collaborative basis.
Based on your project the CTM will suggest an optimal strategy (microinjection, electroporation, viral infection) and choice of reagents (naked DNA, CRISPR/Cas, viral vectors).
Design of Customized Transgenes and Conditional Alleles.
Design and Generation of Gene Targeting Strategies for CRISPS/Cas.
Optimization of Mouse Genotyping Protocols.
Mouse Colony Management and Breeding Strategy Design.
Transgenic Model Generation Services (DPI & ROI)
After designing your targeting strategy, we provide two types of services; our “all-inclusive” Design Production and Injection (DPI) service, and a more budget-friendly version the Rapid Oocyte Injection (ROI) service.
What you provide
Provide us with sequences of desired modification and locus to allow us to optimally support you.
The type of template DNA (or alternative substrate for microinjection) will depend on the strategy chosen. In many cases, it is preferable for the CTM to order the appropriate template based on your sequence information (additional costs will be charged).
DPI: Design, Production & Injection
The complete package
DPI
Our DPI service includes everything from designing guide RNAs and repair templates until the delivery of confirmed transgenic offspring. This includes all services provided with our ROI service plus the following additional services:
ROI: Rapid Oocyte Injection
The “pay as you go” version
ROI
Our ROI service allows you to pay for each injection series. This service demands more effort from your side, as the genotyping and sequencing will be performed by you.
| DPI | What we provide | ROI |
|---|---|---|
| Embryo Injection/Electroporation & Transfer | ||
| Genotyping Service | ||
| Delivery of Selected Offspring | ||
| Guaranteed Delivery of Transgenics | ||
| Fixed Price |
Design Production & Injection
for Uni Basel researchers
- Full guaranteed service – from design to genotyped offspring!
Rapid Oocyte Injection
for Uni Basel researchers
- Price per injection day/series (without success guarantee)
Chimera Generation Service (Chi)
Despite the development of novel genome engineering tools that allow rapid generation of mice carrying desired mutations, extensive ES cell repositories (KOMP, EUCOMM, genetrap) offer the opportunity to generate useful mouse models. In order to facilitate this, CTM offers a ES cell microinjection service.
What we provide
Upon arrival at CTM the ES cells will be injected into at least 80 blastocysts of the appropriate recipient strain (these will vary depending of ES cell type).
The surviving embryos will be implanted into pseudopregnant foster mothers.
Any resulting chimeras will be turned over to the researcher.
What you provide
The researchers must order the selected clones from a repository at their own expense, and expand them to achieve a sufficient number of cells. The ES cell clone to be injected must be brought to the CTM as a live cell suspension on the day of microinjection.
ES clones originating from various repositories do not always produce germline competent chimeras, therefore CTM cannot offer any guarantees as to the number of pups or the number of positive pups that will be obtained. In order to increase chances of success, the CTM recommends the user to import and inject several clones targeting the same allele whenever possible.
Chimera Generation Service
for Uni Basel researchers
- Price per injection day/series (without success guarantee)
Any prices displayed refer to internal (University of Basel) pricing. Please contact us for external pricing.
